Porous Alginate Scaffolds Designed by Calcium Carbonate Leaching Technique

One of the main challenges in modern tissue engineering is to design biocompatible scaffolds with finely tuned porous architecture and capacity load bioactive molecules that guide growth differentiation cells during reconstruction. This work proposes a strategy alginate (PAS) well-tuned by leaching sacrificial vaterite CaCO3 microspheres packed alginate. Pore size interconnectivity depend on sphere dimensions packing as well concentration. Varying these parameters, almost hundred percent pore (or, contrast, zero interconnectivity) can be achieved. Junctions between interconnected pores are about 50–70% provides molecular transport through PASs potentially ensuring diffusion nutrition, oxygen metabolic products when cell seeding. An opportunity fabricate multifunctional scaffold demonstrated encapsulation desired macromolecules into individual (is illustrated dextran loading). Mechanical properties found typical for soft hydrated structures (Young's modulus 19 ± 15 kPa) which appropriate The three lines (HeLa, HEK293, L929) cultured different examine viability adhesiveness.